Human Recombinant SIRP alpha (from HEK293 Cells)

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Conjugation:
Unconjugated
Protein Function:
Immune Checkpoint Proteins
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 1.0 EU per μg by the LAL method.
Biological Activity:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human CD47, Fc Tag (Cat. No. CD7-H5256) at 2 µg/mL (100 µl/well) can bind Human SIRP alpha, His Tag (Cat. No. SIA-H5225) with a linear range of 16-250 ng/mL (QC tested).; FACS assay shows that recombinant Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/ml (Routinely tested).; FACS analysis shows that the binding of Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 1 μg/ml. IC50=0.2257 μg/ml (Routinely tested).
Protease-free:
Y
Animal-Free:
Y
Protein Synonyms:
MYD1|PTPNS1|CD172A|SHPS1|P84|SIRPA|BIT|MFR
UniProtKB:
P78324
Protein/Peptide Name:
SIRP alpha
Purity:
>95% as determined by SDS-PAGE.
Molecular Weight:
38.1 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Shipping Temperature:
Powder,RT