Human Recombinant PD-1 (from HEK293 Cells), Fc Tag

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Conjugation:
Unconjugated
Protein Function:
Immune Checkpoint Proteins
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Tag sequence:
Fc Tag
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 0.1 EU per μg by the LAL method.
Biological Activity:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, His Tag (Cat. No. PD1-H5229) at 5 μg/mL (100 μL/well) can bind Human PD-1, Fc Tag (Cat. No. PD1-H5257) with a linear range of 0.625-20 μg/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Fc Tag, Avi Tag (Cat. No. PD2-H82F6) with a linear range of 0.5-16 ng/mL. (Routinely tested).; Flow Cytometry assay shows that recombinant Human PD-1, Fc Tag (Cat. No. PD1-H5257) can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 μg/mL (Routinely tested).
Protease-free:
Y
Animal-Free:
Y
Protein Synonyms:
CD279|PDCD1|PD1|SLEB2
UniProtKB:
Q15116
Protein/Peptide Name:
PD-1
Purity:
>95% as determined by SDS-PAGE.
Molecular Weight:
42.6 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Shipping Temperature:
Powder,RT