Human Recombinant PD-L1 (from HEK293 Cells) His Tag

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Conjugation:
Unconjugated
Protein Function:
Immune Checkpoint Proteins
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Tag sequence:
His Tag
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 1.0 EU per μg by the LAL method.
Biological Activity:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb (Human IgG1) with a linear range of 0.1-3 ng/mL (QC tested).; Biotinylated Human PD-1, Fc Tag (Cat. No. PD1-H82F1) at 1 μg/mL (5 μL/well) can bind Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) with a linear range of 0.02-0.625 μg/mL as determined in a alphaLISA homogeneous assay (Routinely tested).; Human PD-1, Fc Tag (Cat. No. PD1-H5257) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) with an affinity constant of 3.6 μM as determined in a SPR assay (Biacore T200) (QC tested).; Anti-Human PD-L1 Mab (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 0.286 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protease-free:
Y
Animal-Free:
Y
Protein Synonyms:
B7-H1|PD-L1|CD274|PDCD1L1|PDCD1LG1
UniProtKB:
Q9NZQ7
Protein/Peptide Name:
PD-L1
Purity:
>98% as determined by SDS-PAGE.
Molecular Weight:
26.0 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Shipping Temperature:
Powder,RT