Human Recombinant Her2 (from HEK293 cells)

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Specifications

Conjugation:
Unconjugated
Protein Function:
Bio-Markers & CD Antigens
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 1.0 EU per μg by the LAL method.
Biological Activity:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 0.05 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.2-3 ng/mL (QC tested).; Herceptin (Trastuzumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 1.07 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; Immobilized Herceptin on AHC Biosensor, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 0.93 nM as determined in BLI assay (Fortebio Octet 96) (Routinely tested).
Protease-free:
Y
Animal-Free:
Y
Protein Synonyms:
NEU|MLN19|TKR1|ERBB2|CD340|HER2|HER-2/neu|NGL
UniProtKB:
P04626
Protein/Peptide Name:
Her2
Purity:
>95% as determined by SDS-PAGE.
Molecular Weight:
70.2 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Shipping Temperature:
Powder,RT