Specifications
- Antibody Type:Primary
- Antigen Symbol:XAF1
- Clonality:Polyclonal
- ELISA:Yes
- Host:Rabbit
- ImmunoFluorescence:Yes
- Western Blot:Yes
- Size:100 µg
- Storage Temperature:Store vial at –20 °C. Aliquot contents and freeze at –20 °C. Centrifuge product if not completely clear after standing at room temperature. Stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
- Shipping Temperature:Dry Ice
- Purification:Affinity purified
- Cat. No.:75935-484
- Supplier no.:600-401-FZ7
Specifications
About this item
XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.
Anti-XAF-1 Antibody has been tested for use in ELISA, Western Blotting and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 35 kDa in Western Blots of specific cell lysates and tissues.
Type: Primary
Antigen: XAF1
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Rabbit
Isotype:
Reactivity: