Specifications
- Antibody Type:Primary
- Antigen Symbol:THEM4
- Clonality:Polyclonal
- ELISA:Yes
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Western Blot:Yes
- Size:100 µg
- Storage Temperature:Store vial at –20 °C. Aliquot contents and freeze at –20 °C. Centrifuge product if not completely clear after standing at room temperature. Stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
- Shipping Temperature:Dry Ice
- Purification:Affinity purified
- Cat. No.:75932-864
- Supplier no.:600-401-FE0
Specifications
About this item
THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.
Anti-THEM4 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 27 kDa in Western Blots of specific cell lysates and tissues.
Type: Primary
Antigen: THEM4
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Rabbit
Isotype:
Reactivity: