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Anti-ORAI3 Mouse Monoclonal Antibody [Clone: [1B4F1]]
Anti-ORAI3 Mouse Monoclonal Antibody [Clone: [1B4F1]]
Catalog # 75930-644
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Anti-ORAI3 Mouse Monoclonal Antibody [Clone: [1B4F1]]
Catalog # 75930-644
Supplier Number:  200-301-DH3
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Specifications

  • Antibody Type:
    Primary
  • Antigen Symbol:
    ORAI3
  • Clonality:
    Monoclonal
  • Clone:
    [1B4F1]
  • ELISA:
    Yes
  • Host:
    Mouse
  • Isotype:
    IgG2b
  • Reactivity:
  • Western Blot:
    Yes
  • Size:
    100 µg
  • Environmentally Preferable:
  • Storage Temperature:
    Store vial at –20 °C. Aliquot contents and freeze at –20 °C. Centrifuge product if not completely clear after standing at room temperature. Stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
  • Shipping Temperature:
    Dry Ice
  • Purification:
    Protein A purified
  • Cat. No.:
    75930-644
  • Supplier no.:
    200-301-DH3

Specifications

About this item

Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

Anti-ORAI3 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 31 kDa in Western Blots of specific cell lysates and tissues.

Type: Primary
Antigen: ORAI3
Clonality: Monoclonal
Clone: [1B4F1]
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: