Borate buffered saline (BBS) is commonly used in coating procedures for ELISA.

• Borate buffered saline - BBS
• Aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers
• Tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria

BBS can also be used as a component in blocking solutions for Western blotting. The buffer can be used as a diluent in biological samples and for various applications within biology and biochemistry to maintain the pH.

Application Note: BBS is isotonic and has a strong bactericidal effect. It can be used to dilute solutions for coating procedures. Additives such as Tween 20 and Blotto can be used to add functionality as a washing buffer or blocking buffer.