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Specifications
- Description:MCF-7 Cell Nuclear Extract
- Size:200 µg
- Shipping Conditions:Dry Ice
- Cat. No.:75929-880
- Supplier no.:W09-001-A85
Specifications
About this item
MCF-7 Cell Nuclear Extract
- Ready-to-use lysates
- positive controls
- nuclear lysates
Ready-to-use nuclear extracts produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired add reducing agent prior to heating. The recommended loading volume per lane is 10-30 l depending on the size format of your gel.