Lysis Buffer, 1X, Rockland Immunochemicals

1X Lysis Buffer

• 50 mM Tris Cl, 150 mM Sodium Chloride, 1 mM EDTA, 1% TRITON-X-100, pH 7.4.
• No further preparation is required prior to use. Chill to 2 to 8 °C prior to use.

Lysis Buffer is used to lyse cells under nondenaturing conditions. Cell Lysis Buffer is ideal to assist in Immunoprecipitation which allows for the purification by immunoprecipitation of recombinant proteins containing an epitope tag provided by the user. The user is able to further characterize the resultant purified protein by size, post-translational modification, western blot and other assays.

Chill to 2 to 8 °C prior to use. Lysis Buffer is provided as a ready-to-use 1X solution containing 50 mM Tris Cl, 150 mM Sodium Chloride, 1 mM EDTA, 1% TRITON-X-100, pH 7.4. No preparation is required prior to use.