Recombinant Human IL-1-beta control protein
- Control Protein
- PCA Assays
- ImmunoBlot
Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendritic cells (DCs). IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant human IL-1β is a non-glycosylated protein, containing 153 amino acids, with a molecular weight of 17.4 kDa.
Interleukin-1-beta Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Interleukin-1-beta in immunological assays.
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Storage Conditions:Store vial at 4 °C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at –20 °C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Endotoxin Content:Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.
- Biological Activity:The activity is determined by the stimulation of D10S.G4.1 cells and is typically less than 12 pg/mL.
- Reconstitution Instructions:Restore with deionized water (or equivalent)
- Animal-Free:Y
- Protein Synonyms:EP|Catabolin|LAF|LEM|MCF|Interleukin-1 beta
- Protein/Peptide Name:IL-1-beta
- Purity:>97%
- Formulation:Lyophilized
- Shipping Temperature:Ambient