IL-6 Human Recombinant control protein

• Control Protein
• PCA Assays
• ImmunoBlot

Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contains four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist: the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha-helix is important to the biological activity of the molecule. Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Human IL-6 produced in E.coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21,000 Daltons.

This product is lyophilized from a 10 mM acetic acid solution. For easiest restoration, it is recommended to restore with 1000 µl of 10 mM HCL for a stock concentration of 0.1 mg/ml. This solution can be further diluted in deionized water or other buffers as required. Interleukin-6 (IL-6) is suitable as a control for polyclonal or monoclonal anti-Interleukin-6 (IL-6) in immunological assays.