The Soluble Semaphorin 4D ELISA is for direct determination of soluble Semaphorin 4D in plasma.

• Sensitivity: LOD: (0 pmol/l + 3 SD): 12 pmol/l; LLOQ: 31 pmol/l
• Dynamic Range: 62.5 to 2000 pmol/l
• Sample Size: 100 μl
• Sample Type: Serum
• Incubation Time: 3.5 hours

Semaphorin 4D (SEMA4D or CD100) is a member of a family of transmembrane and secreted proteins that regulates key cellular functions and is involved in cell-cell communication (1-3). Most of the effects of SEMA4D is mediated by plexins (4, 5). SEMA4D participates in numerous physiological processes such as axon guidance, immune regulation, angiogenesis, tumor progression, and bone metabolism (6-9).

Cleavage of SEMA4D near the cell membrane through matrix metalloproteinases leads to the biologically active soluble SEMA4D with a molecular weight of 120 kD consisting of 713 amino acids (3, 5, 10). SEMA4D has emerged to a novel therapeutic target in cancer and in bone diseases (11, 12). Semaphorin 4D is widely studied for its role in neural connectivity, vascularization, cell migration, the immune response, tumor progression, and bone remodeling.