Mouse Recombinant REN1/Renin-1 Protein (His Tag) (from HEK293 Cells)

A DNA sequence encoding the pro form of mouse REN1 (NP_112469.1) (Met 1-Arg 402) was fused with a polyhistidine tag at the C-terminus.

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Mouse
Storage Conditions:
–20…–80 °C
Endotoxin Content:
<1.0 EU per μg of the protein as determined by the LAL method
Biological Activity:
1. Measured by its ability to cleave the fluorogenic peptide substrate 5FAM/QXL™ 520 (PetiPeterdi, J. et al., 2009, Physiology 24:88.). The specific activity is >20 pmoles/min/μg.
2. Immobilized mouse REN1-His (Cat: 50279-M08H) at 10 µg/ml (100 μl/well) can bind biotinylated human AGT-His (Cat: 10994-H08H) with a linear range of 31.25 - 250 ng/ml.
Gene ID:
NP_112469.1
Reconstitution Instructions:
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Carrier-Free:
Y
Animal-Free:
Y
Protein Synonyms:
Ren1c|Ren-A|Ren1d|Rnr|Ren-2|D19352|Ren-1|Rn-1|Ren2|Rn-2|Ren-B|Ren|Ren1
Protein/Peptide Name:
REN
Purity:
>97% as determined by SDS-PAGE.
Molecular Weight:
The recombinant mouse REN1 consists of 392 amino acids and has a predicted molecular mass of 43.2 kDa. As a result of glycosylation, the apparent molecular mass of rmREN1 is approximately 48-55 kDa in SDS-PAGE under reducing conditions.
Sequence:
Met1-Arg402
Shipping Temperature:
Ambient