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Specifications
- Concentration:15 µM
- Application:
- Description:RtcB ligase
- Environmentally Preferable:
- Cat. No.:103307-256
- No. of reactions:25
- Supplier no.:M0458S
Specifications
About this item
RtcB Ligase is expressed as a His-tagged fusion in E. coli.
- This is an Enzyme for Innovation (EFI)
- These enzymes have interesting properties and unique specificities
RtcB Ligase from E. coli joins single stranded RNA with a 3'-phosphate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl. Ligation requires both GTP and MnCl₂ and proceeds through a 3'-guanylate intermediate. For substrates with a 2',3'-cyclic phosphate end, hydrolysis to a 3'-phosphate precedes 3' end activation with GMP and ligation.