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Specifications
- Source:
- Species:
- Size:25 Reactions
- Environmentally Preferable:
- Enzyme Name:KLD
- Cat. No.:103501-322
Specifications
About this item
KLD Enzyme Mix is a unique blend of Kinase, Ligase and DpnI enzymes.
- Degradation of template DNA by DpnI
- Also available as a kit: with and without competent cells
This formulation allows efficient phosphorylation, intramolecular ligation/circularization and template removal in a single 5 minute reaction step at room temperature.
This master mix is a component of the Q5 Site-Directed Mutagenesis Kits and it has been designed for use with fragments that have been PCR-amplified by Q5 Hot Start High-Fidelity DNA Polymerase.