Specifications
- Description:NF-κB (human p50) Transcription Factor Assay Kit
- Cat. No.:75816-692
- No. of wells:96
- Contents:Kit contains: Assay Buffer, Reagents, Positive Control, Antibody Binding Buffer, Primary Antibody, Buffer Concentrate, Polysorbate 20, NF-kB Competitor dsDNA, Goat Anti-Rabbit HRP Conjugate, 96-well Strip Plate, 96-well Cover Sheet, Developing Solution, and Stop Solution
- Supplier no.:10006912-96
Specifications
About this item
A 96-well assay for measurement of NF-κB (human p50).
- A sensitive, non-radioactive method of detecting NF-κB (p50) from whole cell lysates
- 96-well plate format replaces EMSAs
- Capture the transcription factor using a specific dsDNA sequence bound to the plate
- Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
Nuclear Extraction Kit available to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates.
Cayman’s NF-κB (human p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element.
NF-κB (p50) is detected by addition of specific primary antibody directed against NF-κB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
The Cayman Chemical NF-κB (human p50) Transcription Factor Assay detects human NF-κB (p50). It will not cross-react with NF-κB (p65).