To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Enzyme type:Recombinant
- Source:E. coli
- Species:Human
- Size:100 µg
- Environmentally Preferable:
- Storage Conditions:–80 °C
- Shipping Conditions:–80 °C, dry ice
- Enzyme Name:STING AQ variant
- Enzyme Synonyms:Endoplasmic Reticulum Interferon Stimulator, Stimulator of Interferon Genes, TMEM173
- Purity:≥80% estimated by SDS-PAGE
- Molecular Weight:28.8 kDa
- Formulation:50 mM HEPES, pH 8.0, 150 mM sodium chloride, and 10% glycerol
- Cat. No.:76293-156
Specifications
About this item
An active, pure human recombinant enzyme.
STING AQ variant (human recombinant) contains amino acids 138-379 of the wild-type STING variant (R232) with an alanine substituted for glycine at position 230 and a glutamine substituted for arginine at position 293. Stimulator of interferon genes (STING) is a component of the innate immune response that binds to cyclic dinucleotides, which are bacterial second messengers. Recognition of cyclic-di-GMP (c-di-GMP), c-di-AMP, or c-GMP-AMP leads to activation of NF-κB and transcription of immunomodulatory genes, including type I interferons (IFN). The R232 variant of STING is the most common variant in the human population, found at a frequency of 57.9% in the 1000 Genome Project.{38697} The SNP variant H232 is found at a 13.7% frequency. The G230A, R293Q double mutation occurs in 5.2% of the human population and when expressed in HEK293T cells, this mutation reduces the IFN response to bacterial ligands by 30-40% compared to wild-type STING. Whereas the R293Q substitution alone is severely defective in response to bacterial cyclic dinucleotides, the G230A substitution is thought to help maintain some ability of this variant to respond to bacterial cyclic dinucleotides. Based on crystal structure of STING, the G230A mutation alters the conformation of the lid region that clamps onto the c-di-GMP, however, the R293 residue does not directly bind to c-di-GMP. The R293Q mutation may instead disrupt STING activity indirectly by altering the function of a nearby cysteine residue required for IFN-β expression.
Used for: Infectious Disease, Autoimmunity
Endotoxin level and content: Not tested