ECM 2001+ instruments offer best-in-class features for cell fusion and transfection applications.

• AC waveform of 0.2 - 2.0 MHz
• Square wave mammalian electroporation capabilities
• Wide range of voltages from 5 V to 3000 V
• Pre-set protocols include most common cell fusion applications
• User-defined protocols allow unlimited ability to add and modify protocols
• Advanced programmability, combines up to 19 AC steps pre- and post- DC fusion step
• Simple to use, large 7" touchscreen interface

The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.

Fast, efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications is facilitated by the combination of AC and DC wave pulses. Fusion is achieved by the generation of an AC current wave form that generates a benign dielectrophoretic alignment of cells. With a 30 ms switchover time from AC to DC, efficient fusion takes place. After fusion, the AC is reapplied maintaining the cell compression for the rounding off process, resulting in a higher number of hybrids.

For use in large-scale hybridoma production applications, the ECM 2001+ generator, 2 ml coaxial optimization or 9 ml coaxial production chambers, high voltage output cable, and female/female adapters may be used. Alternatively, for medium-scale hybridoma production applications, the ECM 2001+ generator may be combined with 650 ml, 3.2 mm gap or 2 ml, 10 mm gap microslides, high voltage output cable, and micrograbber adapters. This system does not require proprietary fusion medium, however may be used with the Cytofusion Medium C for enhanced efficiency, reproducibility, and convenience.