- Primary antibody reactivity:Rat
- Description:Multi-Neurotrophin Rapid Screening ELISA Kit: Rat
- Size:48 Tests
- Cross Reactivity:This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the human and mouse form of each neurotrophin, but with varying efficiency depending on the target protein
- Detection Range:NGF (3.9 - 250 pg/ml) BDNF (7.8 - 500 pg/ml) NT3 (15.6 - 1000 pg/ml) NT4/5 (15.6 - 1000 pg/ml)
- Sensitivity:Typical LODs achieved based on 150% of background OD: NGF: 3 pg/ml BDNF: 1 pg/ml NT3: 4 pg/ml NT4/5: 10 pg/ml
- Cat. No.:75838-800
- Supplier no.:BEK-2232
The biosensis® Multi-Neurotrophin RapidTM Screening ELISA kit has been designed to allow rapid screening and quantification of rat NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum.
This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed.
The Multi-Neurotrophin RapidTM Screening ELISA kit therefore presents a cost-effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded.