Rapid™ GDNF ELISA Kit: Rat
About this item
The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples.
This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. A GDNF positive control (QC sample) is provided to assure consistent assay performance.
This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications.
Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples.
Delivery information: The ELISA kit box contains 1 or 2×96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Caution: It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
2 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples.
This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. A GDNF positive control (QC sample) is provided to assure consistent assay performance.
This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications.
Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples.
Delivery information: The ELISA kit box contains 1 or 2×96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Caution: It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 75838-794
- 75838-796
- Target protein:
- GDNF
- GDNF
- Primary antibody reactivity:
- Rat
- Rat
- Assay Type (ELISA with LOV):
- Sandwich
- Sandwich
- Sensitivity:
- Typical limit of detection (LOD) for rrGDNF is <15 pg/ml determined as blank value plus 3× standard deviation of blank OD (n=10)
- Typical limit of detection (LOD) for rrGDNF is <15 pg/ml determined as blank value plus 3× standard deviation of blank OD (n=10)
- Detection Range:
- 7.8 - 500 pg/ml
- 7.8 - 500 pg/ml
- Cross Reactivity:
- The antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF RapidTM ELISA kit. All Biosensis GDNF RapidTM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF RapidTM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF RapidTM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/ml in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin
- The antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF RapidTM ELISA kit. All Biosensis GDNF RapidTM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF RapidTM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF RapidTM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/ml in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin
- Specificity:
- Rat. The antibodies used in this kit detect mouse and human GDNF.
- Rat. The antibodies used in this kit detect mouse and human GDNF.
Specifications
Product Family Options
Product Information
- SizeAvailabilityPrice
- 75838-794GDNF Rapid ELISA Kit: Rat (1 plate)Supplier Number: BEK-2230-1PItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.75838-794Target proteinGDNFPrimary antibody reactivityRatAssay Type (ELISA with LOV)SandwichSensitivityTypical limit of detection (LOD) for rrGDNF is <15 pg/ml determined as blank value plus 3× standard deviation of blank OD (n=10)Detection Range7.8 - 500 pg/mlCross ReactivityThe antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF RapidTM ELISA kit. All Biosensis GDNF RapidTM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF RapidTM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF RapidTM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/ml in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and PersephinSpecificityRat. The antibodies used in this kit detect mouse and human GDNF. - 75838-796GDNF Rapid ELISA Kit: Rat (2 plates)Supplier Number: BEK-2230-2PItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.75838-796Target proteinGDNFPrimary antibody reactivityRatAssay Type (ELISA with LOV)SandwichSensitivityTypical limit of detection (LOD) for rrGDNF is <15 pg/ml determined as blank value plus 3× standard deviation of blank OD (n=10)Detection Range7.8 - 500 pg/mlCross ReactivityThe antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF RapidTM ELISA kit. All Biosensis GDNF RapidTM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF RapidTM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF RapidTM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/ml in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and PersephinSpecificityRat. The antibodies used in this kit detect mouse and human GDNF.
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