The Biosensis NGFR/p75ECD RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75ECD in less than 4 hours n cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a goat anti- p75ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards.

A mouse p75ECD positive control (QC sample) is provided to assure consistent assay performance. This NGFR/p75ECD ELISA kit employs a recombinant mouse p75ECD-Fc chimera protein as standard. While there is a current lack of a true mouse p75ECD standard, this ELISA kit allows quantification of mouse p75ECD as p75ECD–Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75ECD. This kit has not been tested for other applications.