ABLE Competent Cells for toxic clones reduce copy number four to ten fold compared to the XL1-Blue strain.
- Reduces the copy number of ColE1-derived plasmids (such as pUC and pBluescript® plasmids) four-fold
- Decreases level of cloned protein product to increase cell viability
- Transformation efficiency of 5×10⁶ to 2×10⁷ transformants/µg
- Also available in an electroporation-competent version
ABLE C Competent Cells for toxic clones enhance the probability of cloning DNA encoding toxic proteins. By reducing the copy number of ColE1-derived plasmids, the ABLE C strain decreases the levels of cloned gene products and enhances the probability of propagating a toxic clone.
The ABLE C Competent Cells provide a viable alternative to commonly used cloning vectors with high copy numbers. Positive clones observed on initial screening of lambda libraries can be excised or re-cloned into any convenient vector and can be introduced into ABLE C strains.
ABLE K Competent Cells for toxic clones enhance the probability of cloning DNA encoding toxic proteins. By reducing the copy number of ColE1-derived plasmids, the ABLE K strain decreases the levels of cloned gene products and enhances the probability of propagating a toxic clone.
ABLE K Competent Cells provide a viable alternative to commonly used cloning vectors with high copy numbers. Positive clones observed on initial screening of lambda libraries can be excised or re-cloned into any convenient vector and can be introduced into ABLE K strains.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.