About this item
Native mouse nerve growth factor (2.5S) protein purified from submaxillary glands
Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF.
Specifications
- Conjugation:Unconjugated
- Protein Function:Neurotrophin, growth factor
- Protein/Peptide Type:Native
- Source:Submaxillary glands
- Species:Mouse
- Storage Conditions:Store lyophilized protein at 2-8C. After reconstitution, store at 2-8C short term. Store long-term at –20 °C to -80C. Avoid repeated freezing and thawing. See expiration date for shelf-life estimates, actual times may vary depending upon experimental conditions and laboratory handling.
- Biological Activity:Tested by stimulation of 8 day old chick embryo dorsal root ganglia or neurite outgrowth in rat PC12 cells.
- Gene ID:P01139 NGF_MOUSE
- Reconstitution Instructions:Reconstitute with the addition of 500 µL sterile water and divide into single use aliquots. Concentration after reconstitution: 1 mg/mL
- Protein Synonyms:beta NGF|2.5S NGF
- Protein/Peptide Name:NGF
- Purity:Greater than 90% (as determined by SDS electrophoresis)
- Molecular Weight:The homodimer has a molecular weight of ~26KDa and the monomer ~13KDa as confirmed by SDS PAGE.
- Formulation:Lyophilised from PBS, pH 7.4 without preservatives.
- Shipping Temperature:ambient