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Specifications
- Antibody Type:Primary
- Antigen Name:Microtubule-associated protein 1 light chain 3A
- Antigen Symbol:MAP1LC3A
- Clonality:Monoclonal
- Clone:BS405
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2a kappa
- Reactivity:Human,Rat
- Western Blot:Yes
- Epitope:A synthetic peptide (C-RSFADRCKEVQQI) corresponding to amino acids 11-23 of human MAP1LC3 A protein was conjugated to a carrier protein and the complex used as the immunogen. This human sequence is homologous with mouse and rat MAP1LC3 A.
- Format:Protein G purified IgG.
- Form:Lyophilized
- Gene ID:Q9H492 MLP3A_HUMAN
- Antigen Synonyms:MAP1 light chain 3-like protein 1|Microtubule-associated proteins 1A/1B light chain 3A|Autophagy-related ubiquitin-like modifier LC3 A|MAP1LC3A|APG8a|Microtubule-associated protein 1 light chain 3 alpha|Autophagy-related protein LC3 A|MAP1A/MAP1B LC3 A|MAP1A/1B light chain 3 A
- Storage Buffer:PBS, pH 7.4, with 3% Trehalose, without preservatives
- Sequence:C-RSFADRCKEVQQI
- Shipping Temperature:Room (ambient) temperature.
- Immunogen:A synthetic peptide (C-RSFADRCKEVQQI) corresponding to amino acids 11-23 of human MAP1LC3 A protein was conjugated to a carrier protein and the complex used as the immunogen. This human sequence is homologous with mouse and rat MAP1LC3 A.
- Purification:Protein G purified.
- Cat. No.:76265-486
Specifications
About this item
MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes. Antibody reacts with human and rat. The antibody is expected to react with mouse MAP1LC3A protein due to 100% sequence homology.
Flow Cytometry (20 µg/mL), Western blot (10 µg/mL), Immunocytochemistry (1-2 µg/mL). Other applications have not yet been tested. Biosensis recommends optimal dilutions and concentrations should be determined by the end user.
Type: Primary
Antigen: MAP1LC3A
Clonality: Monoclonal
Clone: BS405
Conjugation: Unconjugated
Epitope: A synthetic peptide (C-RSFADRCKEVQQI) corresponding to amino acids 11-23 of human MAP1LC3 A protein was conjugated to a carrier protein and the complex used as the immunogen. This human sequence is homologous with mouse and rat MAP1LC3 A.
Host: Mouse
Isotype: IgG2a, k-light chain
Reactivity: Human, Rat