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- Description:Amylo-Glo RTD Amyloid Plaque Stain Reagent with EtBr counter stain 10x
- Environmentally Preferable:
- ImmunoFluorescence:Yes
- Storage Temperature:The stock solution can be stored for up to 6 months at 4 °C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.
- Concentration:10X
- Formulation:Liquid
- Shipping Temperature:Ambient
- Cat. No.:76264-658
Amylo-Glo® RTD™ 'Ready to Dilute' Staining reagent is designed to stain amyloid plaques in tissue sections.
- For Amyloid plaques both intraneuronal and vascular for Amylo-Glo
- Very bright blue UV excitable stain under physiological conditions
- Unique excitation/emission profile and mild staining
- Combination for multiple immunofluorescent labeling studies
- Storage Up to six months at 4 °C
The Biosensis AG-400-AG kit utilizes an ethidium bromide counter stain for a quick and effective way to visualize cell nuclei and cell bodies of cells while under UV illumination allowing the assessment of amyloid plaques and cell/tissue positioning as well in one step. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120– 126). Using Amylo-Glo® results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo® RTD™ is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo® fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol). Reagent stains amyloid plaques both intraneuronal and vascular for Amylo-Glo. Ethidium bromide stains nuclei and cell bodies, used as both DNA and RNA label.
Staining of amyloid plaques in human and animal tissues, see included protocol. EtBr counter stain stains nuclei and cell bodies for easy identification and spacial orientation. Excitation Peak: 334; Emission Peak: 533 nm - unbound, 438 nm when bound to amyloid. To visualize Amylo-glo in tissue, UV light is required. For example, Amylo-Glo tissue can be examined using an epifluoresent microscope with UV (Nikon UV-2A) filter cube. Excitation (325 to 375 nm) Emission (400 to 450 nm) is typical. Also note, it is not uncommon for Amylo-Glo to appear light yellow when examined by eye, yet appear a light blue color when photographed.
Visualization of EtBr: Ethidium bromide has an excitation peak of 300 nm and an emission peak 595 nm. Most UV compatible filter sets can be used.