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Specifications
- Size:1 mg
- Storage Conditions:4 °C, Do NOT freeze!
- Protein/Peptide Name:SMCC Activated B - PE
- Shipping Temperature:Shipped in Blue Ice
- Cat. No.:103010-440
- Supplier no.:AS-72109
Specifications
About this item
B-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, is isolated from cyanobacteria and eukaryotic algae. SMCC activated B-PE is chemically modified with SMCC. SMCC reacts with the primary amine on B-PE and introduces maleimide groups to B-PE. These maleimide groups easily react with thiol groups of target protein without the need for any additional activation, resulting in convenient conjugation of B-PE with proteins. Its primary absorption peak is at 545 nm with secondary peak at 563 nm. B-PE and the closely related R-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. B-PE conjugates have been widely used in applications such as flow cytometry and multi-color immunofluorescent staining.
- Absorbance: Abs/Em = 545/575 nm