Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.

• Grade : Molecular Biology Reagent
• Specific Activity : ≥70 Kunitz units/mg
• Key Applications : Purification of DNA; Analysis of RNA sequences; RNase protection assays; Hydrolyze RNA contained in protein samplesApplication Areas
• Product Type : Proteins, Enzymes & Peptides
• Extinction Coefficient : (E1%) E1%280 = ~7.3
• Presentation : White Powder
• Format : Lyophilized powder, Salt-free and protease-free
• Isoelectric point (pI) : 9.45
• pH Optimum pH: 7.0 to 7.5
• NOTES : Inhibitors: Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid. Composition: The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure indicates that flexibility in a three-dimensional structure is essential for optimal catalytic activity.
• Specificity : The enzyme is specific for pyrimidine nucleoside linkages, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
• Solubility: Dissolves readily at 2 mg/ml in analytical grade water to give a clear colorless solution.