About this item
The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.
- MgCl2 and dNTPs are included at optimized concentrations
- Master Mix is stable after multiple freeze thaws, reducing wastage and increasing batch-to-batch reliability
- Start Taq polymerase enzyme requires only three minutes for activation
- The dye is non-toxic and can be added at saturating concentrations - insuring minimal inhibition while preserving high sensitivity
- Master Mix can be used with the AriaMx Real-Time PCR System or any HRM-capable thermal cycler
The Master Mix combines a mutant Fast-Start Taq polymerase, optimized concentrations of MgCl2 and dNTPs, and EvaGreen, release-on-demand dye to enable researchers to mix-and-go faster and with total confidence - even for the most difficult genotypes.
The optimized MgCl2 and dNTP concentrations reduce assay preparation time, while low surfactant levels minimize bubble trouble during pipetting. In addition, the 12-month storage at -20 °C is more than double that of other HRM reagents.
The passive reference dye may be added to fluorescence-based quantitative PCR assays to compensate for non-PCR related variations in fluorescence.
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