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Specifications
- Column Format:Analytical
- Ø int.:4.60 mm
- Particle Size:20 µm
- Pore size:1000 Å
- Description:TSKgel Protein A-5PW
- Length:35 mm
- Column hardware:PEEK
- Cat. No.:103257-544
- Packing material:Hydroxylated Methacrylic Polymer Beads
- Exclusion limit:1000 kDa
- Supplier no.:23483
Specifications
About this item
TSKgel® Protein A-5PW columns have been designed for the rapid separation and robust quantification of a variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection. The column can be used for more than 2000 injections without regeneration or cleaning.
- Packed with hydroxylated methacrylic polymer beads
Designed with a high degree of crosslinking, which allows high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain. An enhanced rProtein A ligand is bound to the TSKgel® 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH.
The wide range loading capacity of the TSKgel® Protein A-5PW column can accurately determine the titer of mAb at various stages of cell culture media processing. The low level of protein A leaching makes this column a good candidate for small scale purification of mAbs for initial characterization.