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- Size:20 μg
- Cat. No.:PAN1501
- Storage temperature:–30...–10 °C
- Supplier no.:N1501
The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System.
- Vectors Containing NanoLuc(R) Luciferase Expressed from Mammalian Promoters
- Multiple promoter options (CMV, TK and PGK) are available to obtain appropriate levels of the control reporter in your experimental system
- Co-transfect with experimental firefly luciferase vectors as normalization control
- Measure signal using the Nano-Glo(R) Dual-Luciferase(R) Reporter (NanoDLR™) Assay System
The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System. NanoLuc luciferase is a small (19.1kDa), stable reporter enzyme that can be up to 100-fold more sensitive than the flash-type Renilla signal in the DLR Assay and more than 3,000-fold more sensitive than the Renilla signal in the Dual-Glo Assay. The increased brightness of the NanoLuc Luciferase means you use less control DNA, minimizing assay artifacts and providing a stable control signal for normalization of the experimental Fluc reporter. The vectors are engineered with minimal consensus transcription factor-binding sites to reduce anomalous expression.