Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
- Size:1 each
- Cat. No.:PAN1381
- Storage temperature:–30...–10 °C
- Supplier no.:N1381
NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.
- Vectors to Measure Intracellular Protein Turnover of HIFA and NRF2
- Ready-to-use constructs are predesigned, optimized and tested for low endotoxin levels
- C-terminal fusion of HIF1A NanoLuc(R) luciferase reporter in pNLF1-HIF1A [CMV/neo] Vector
- NRF2 vector system has a pKEAP1-expressing vector for regulating intracellular NRF2 levels and pNLF1-NRF2 [CMV/neo] Vector expressing a C-terminal fusion with NanoLuc(R) luciferase
The rate of protein turnover is tightly regulated for many signaling proteins involved in oncogenesis and response to cellular stress. Protein stabilization and subsequent accumulation occurs in response to changing cellular conditions resulting in activation of downstream transcriptional events. The NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter to enable stability studies of two key signaling proteins, HIF1A and NRF2, providing a method to directly measure this primary signaling event. HIF1A Vector System: The HIF1A Vector System enables simple quantification of intracellular HIF1A protein levels to study the dynamics of this signaling protein in mediating cellular response to hypoxia. It contains a vector encoding NanoLuc fused to the C-terminus of the HIF1A protein under control of the CMV promoter plus Transfection Carrier DNA to allow for titratable intracellular fusion protein expression. NRF2 Vector System: The NRF2 Vector System enables simple quantification of intracellular NRF2 protein levels to study the dynamics of this signaling protein in mediating cellular response to oxidative stress. It contains a vector encoding NanoLuc fused to the C-terminus of the NRF2 protein under the control of the CMV promoter, a pKEAP1-expressing vector for proper regulation of intracellular NRF2 levels and Transfection Carrier DNA for titratable intracellular fusion protein expression.