These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

• Cloning Vectors with Dual Protein Fusion Tags for E. coli and Cell-Free Protein Expression
• Choose from N- and C-terminal fusion options
• Use either the polyhistidine tag or HaloTag(R) ORF for protein expression and analysis
• Clone an protein coding region using PCR or restriction enzyme digestion

The following vectors are used for inducible expression of HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein. The MCS vectors contain a His6-HaloTag dual tag. The dual tag enables protein purification with the reusable Ni-resin while retaining the HaloTag covalent labeling properties. Multiple Cloning Site (MCS) Vectors: pH6HTN His6HaloTag T7 Vector (Cat.# G7971) is designed for protein expression with an N-terminal His6-HaloTag dual tag in E. coli and T7 cell-free expression systems. pH6HTC His6HaloTag T7 Vector (Cat.# G8031) is designed for protein expression with a C-terminal His6-HaloTag dual tag in E. coli and T7 cell-free expression systems.