pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.

• Vectors That Are Co-transfected into Cells as Controls in Dual-Reporter Assays
• Co-transfect into cells where Renilla or NanoLuc(R) luciferase is the experimental reporter gene
• SV40, PGK and TK promoter options offer different expression levels suitable for most dual-reporter assays

The Promoter-driven firefly control vectors are commonly co-transfected with experimental Renilla or NanoLuc luciferase vectors for use in the Dual-Luciferase or Dual-Glo Reporter Assay Systems. The control firefly vectors should give an almost invariant level of activity, while the experimental Renilla or NanoLuc vector varies with treatment. The pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors offer three different promoter options for your dual-reporter assay.