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Specifications
- Size:20 μg
- Cat. No.:PAE1841
- Storage temperature:–30...–10 °C
- Supplier no.:E1841
Specifications
About this item
Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.
- Constitutively Express Cloned Insert for Transient or Stable Transfection
- Select for stably transfected cells with neomycin phosphotransferase gene
- Increase the steady-state level of RNA with the late SV40 polyadenylation signal approximately fivefold more than the early SV40 polyadenylation signal
- Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate ssDNA in E. coli using the f1 origin of replication
The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418.