The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

• For Expressing Untagged Proteins in Mammalian Cells with Neomycin Selection and Renilla Luciferase
• Offers high-level protein expression from the CMV promoter
• Use Renilla luciferase as a transfection control
• Transfer your cloned insert into other Flexi(R) Vectors for additional applications (e.g., bacterial or cell-free protein expression) as needed

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. The vector below is designed specifically for high-level expression of proteins in mammalian cells from the CMV promoter. The pFN9A Vector provides Renilla luciferase, which may be used as a transfection control. The pFN9A Vector was designed to complement pGL4 firefly luciferase vectors when exogenous proteins (e.g., a receptor of transcription factor) must be expressed for reporter assays. All inserts may be confirmed by cell-free expression with the TNT T7 Quick System (Cat.# L1170). Note: Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.