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- Description:Single-Use JM109 Competent Cells, >10(8)cfu/ug, 1ml
- Size:1ml
- Storage Conditions:Store at –70°C
- Cat. No.:PAL2005
- Supplier no.:L2005
JM109 is a K strain that is recA- and endA- to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening.
- Ready-to-Use Competent E. coli Cells for Recombinant Cloning and Subcloning
- Cells are ready to use—no preparation time necessary
- Transformation efficiencies guaranteed
- No worries about undesirable recombination events (recA- mutation), and no nuclease carryover in miniprep DNA (endA- mutation)
JM109 is a K strain that is recA- and endA- to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 10(8)cfu/ug and Subcloning Efficiency at greater than 10(7)cfu/ug. JM109 Competent Cells are prepared according to a modified procedure of Hanahan. JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (r(k)-, m(k)+), relA1, supE44, Delta(lac-proAB), [F' traD36, proAB, laqI(q)ZDeltaM15].