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- Description:Single Step (KRX) Competent Cells, 20 x 50microl
- Size:20 x 50 μl
- Storage Conditions:Always store competent cells at –70°C. Thaw on ice when ready for use. Do not refreeze thawed, unused aliquots.
- Cat. No.:PAL3002
- Supplier no.:L3002
The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression with one strain.
- Ready-to-Transform E. coli Strain with Tightly Controlled Protein Expression
- Ready for protein expression two days after transformation
- Dramatic control of expressed protein-coding regions with higher protein yields than BL21(DE3)-derived strains
- Convenient blue/white screening for detecting recombinant clones
The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression. These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli. Transformation efficiencies are greater than 10(8) cfu/ug, similar to other highly competent cells. The single step cells are available in both single (50ul) and multiple transformation (200ul) sizes. KRX can also be used for blue/white screening. Single Step (KRX) is an E. coli K strain that contains a chromosomal copy of the T7 RNA polymerase driven by a rhamnose promoter (rhaBAD) to provide dramatic control of the proteins expressed via a T7 promoter. Pre-induced expression protein levels are significantly lower than those of BL21(DE3)-derived strains. This feature facilitates cloning and expression of proteins toxic to E. coli. Genotype: [F', traD36, DeltaompP, proA+B+, lacIq, Delta(lacZ)M15] DeltaompT, endA1, recA1, gyrA96 (Nal(r)), thi-1, hsdR17 (r(k)-, m(k)+), e14- (McrA-), relA1, supE44, Delta(lac-proAB), Delta(rhaBAD)::T7 RNA polymerase.