The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.

• Simplified Transcription/Translation of DNA Cloned in Plasmid or Lambda Vectors with a T7 Promoter
• Express up to 500microg/ml of protein in one hour
• Flexible detection of expressed proteins by Coomassie(R) staining or fluorescence or biotinylated labels

The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for translation. This results in greater stability and enhanced expression of target proteins.