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- Description:pGEM DNA Markers, 50microg
- Size:50 μg
- Storage Temperature:-30°C to -10°C
- Cat. No.:PAG1741
- Supplier no.:G1741
Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These markers are generated from separate digests of pGEM(R)-3 Vector DNA with HinfI, RsaI and AvaII and then combined.
- Fifteen DNA Fragments of 36-2,645bp, Combined From Three Separate Digests of pGEM(R)-3 Vector
- Load 1microl/lane
- Supplied at a concentration of 1µg/microl
- Supplied with 6X Blue/Orange Loading Dye
DNA Digest Markers are created by digesting plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. pGEM DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM-3 Vector DNA with HinfI, RsaI and AvaII later combined to form the markers. Recommended Loading: Load 1microl/lane.