The HaloTag Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.

• Identify more Physiologically Relevant Protein Partners
• Rapid capture of simple and complex protein pairs directly from lysates
• Move from pull-downs to imaging studies using same construct

The HaloTag Mammalian Pull-Down Systems (Cat.# G6500, G6504 and G6509) are designed to capture and purify intracellular binary and higher order protein complexes, including transient or weakly interacting partners. HaloTag Mammalian Pull-Down System (Cat.# G6504) includes buffers and resin necessary to perform a HaloTag pull-down. HaloTag Mammalian Pull-Down and Labeling System (Cat.# G6500) includes everything in G6504 plus the HaloTag TMRDirect Ligand, which allows correlative cellular localization and real-time imaging studies. HaloTag Complete Pull-Down System (Cat.# G6509) includes everything in G6500 plus a starter cloning system, Wizard SV Gel and PCR Clean-Up System, and FuGENE HD Transfection Reagent. The HaloTag Control Vector provides protein expression of the HaloTag protein in mammalian cells, E. coli or in vitro expression systems dependent on human cytomegalovirus (CMV) intermediate early enhancer, T7 or SP6 RNA polymerase promoters. It can be used as a control for any HaloTag experimental system and can be used for both stable and transient HaloTag expression in mammalian cells; for stable expression, co-transfection with a vector containing a selectable marker is required. The Protease Inhibitor Cocktail, 50X, is a mixture of six different protease inhibitors with different target protease specificities. This product is provided in a freeze-dried format and can be reconstituted using either 100% ethanol or DMSO. The Mammalian Lysis Buffer is designed for use with HaloTag Mammalian-based expression systems such as the HaloTag Mammalian Pull-Down and Labeling Systems (Cat.# G6500 and G6504) as well as the HaloCHIP System (Cat.# G9410). Formulation consists of 50mM Tris-HCl, 150mM NaCl, 1% Triton X-100 and 0.1% sodium deoxycholate (pH 7.5).