About this item
Fast RNA detection with these easy-to-use RNA LAMP (RT-LAMP) Master Mixes
- Isothermal Amplification: Facilitates running amplification reactions with simplified instrumentation
- Master Mix Format: Streamlines reaction setup while reducing potential handling errors
- Simplified Optimization: Focuses optimization on the two critical reaction parameters - primer design and reaction temperature
- Lyophilization-ready: Avoids redesign of assays to remove components known to inhibit lyophilization – all components are lyophilization-compatible
The LavaLAMP® RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which the RNA target is first converted into cDNA and then that cDNA acts as template for additional LAMP, all using a single DNA polymerase. By combining the strong activity of this enzyme with an optimized master mix formulation, we’ve produced an easy-to-use system that enables fast, sensitive detection of specific RNA targets.
Most LAMP systems are available as multi-component kits that require optimization of many parameters such as MgSO4, betaine, and enzyme concentrations as well as reaction temperature, primer design and primer concentration. The LavaLAMP RNA Master Mix greatly simplifies reaction optimization by providing a “pre-optimized reaction formulation” allowing you to focus your optimization experiments on the two most important reaction parameters – RNA LAMP primer design and reaction temperature.
It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties. If the purchaser is not willing to accept these use limitations, Lucigen Corporation is willing to accept return of the product for a full refund.
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