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- Application:
- Method / Format:Column (silica-based)
- Prep size:Mega
- Refine target molecule:Plasmid
- Sample type:Bacteria
- Target molecule:Plasmid
- Description:Wizard Plus Megapreps DNA Purification System, 5 preps
- Size:5 preps
- Environmentally Preferable:
- Sample Size:600 mL–1 L of culture
- Description:Wizard Plus Megapreps DNA Purification System
- Yield:>1 mg
- Cat. No.:PAA7300
- Supplier no.:A7300
A simple, reliable, silica-resin-based method for rapid isolation of plasmid DNA from 1 liter cultures.
- More than 1mg Plasmid from 1L Cultures
- 3-hour protocol
- Resin-based batch column method
- No organic extractions
Wizard Plus Megapreps DNA Purification System provides a simple and rapid method for large-scale purifications of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of high-copy-number plasmid DNA (200-20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511).