- Application:RT-qPCR probe
- One step:Yes
- Description:GoTaq Probe 1-Step RT-qPCR System
- Cat. No.:PAA6120
- No. of reactions:80 at 50 µl, 200 at 20 µl, 400 at 10 µl
- Volume:2 ml
- Supplier no.:A6120
GoTaq Probe RT-qPCR Systems enable detection and relative quantification of RNA expression levels.
- Simple, One-Step RT-qPCR for RNA Expression Analysis
- Includes GoScript™ Reverse Transcriptase and GoTaq(R) Probe qPCR Master Mix. RNasin(R) RNase protection provided in the supplied GoScript™ RT Mix
- Compatible with fast and standard cycling methods using probe-based assays
- Sensitive detection for quantitation over a broad dynamic range
The GoTaq Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript Reverse Transcriptase and GoTaq Probe qPCR Master Mix in a single-step real-time amplification reaction. The GoScript RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript Reverse Transcriptase, RNasin Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.