To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Description:BCA Protein Assay Kit
- Brand Name:OZBI
- No. of transfections/assays:2500 assays
- Cat. No.:103549-592
- Volume:500 ml BCA reagent A + 15 ml copper solution B + × 1 ml - 1500 µg/ml BSA
Specifications
About this item
BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content even in the presence of detergents.
This kit is based on the reduction of Cu2+ to Cu1+ by protein in alkaline solution; monovalent copper ions produced are detected in a concentration-dependent manner. Bicinchoninic acid (BCA) chelates with the reduced copper Cu1+ and forms a water-soluble purple reaction complex that exhibits a strong absorbance at 562 nm. Absorbance is linear over a wide range of protein concentrations between 25-2000 µg/mL. In general, protein concentrations are estimated with reference to a commonly used protein standard; the kit also includes Bovine Serum Albumin (BSA) at 1.5 mg/mL as a protein standard for a convenient preparation of protein concentration standard curves.