Soil DNA Extraction Kit, IBI Scientific
About this item
The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples.
- Sample Size: 250-500 mg of soil
- Expectant Yield: up to 5 µg of genomic DNA
- Operation Time: 40 minutes or less
- Format: beadbeating tubes, PCR inhibitor removal columns and genomic DNA spin columns
- Elution Volume: 30 – 100 µl
The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized and disrupted using SL1 (lysis) Buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.
: For research and development purposes only.
3 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples.
- Sample Size: 250-500 mg of soil
- Expectant Yield: up to 5 µg of genomic DNA
- Operation Time: 40 minutes or less
- Format: beadbeating tubes, PCR inhibitor removal columns and genomic DNA spin columns
- Elution Volume: 30 – 100 µl
The Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized and disrupted using SL1 (lysis) Buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.
: For research and development purposes only.
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 75878-320
- 75878-322
- 75878-324
- Description:
- Soil DNA Extraction Kit
- Soil DNA Extraction Kit
- Soil DNA Extraction Kit
- Target molecule:
- DNA
- DNA
- DNA
- Refine target molecule:
- Total DNA
- Total DNA
- Total DNA
- Sample type:
- Soil
- Soil
- Soil
- Method / Format:
- Column (silica-based)|Bead beating
- Column (silica-based)|Bead beating
- Column (silica-based)|Bead beating
- Prep size:
- Mini
- Mini
- Mini
- Yield:
- up to 5 µg of genomic DNA
- up to 5 µg of genomic DNA
- up to 5 µg of genomic DNA
Specifications
Product Family Options
Product Information
- SizeAvailabilityPrice
- 75878-320Soil DNA Extraction KitSupplier Number: IB47800Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.75878-320DescriptionSoil DNA Extraction KitTarget moleculeDNARefine target moleculeTotal DNASample typeSoilMethod / FormatColumn (silica-based)|Bead beatingPrep sizeMiniYieldup to 5 µg of genomic DNA - 75878-322Soil DNA Extraction KitSupplier Number: IB47801Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.75878-322DescriptionSoil DNA Extraction KitTarget moleculeDNARefine target moleculeTotal DNASample typeSoilMethod / FormatColumn (silica-based)|Bead beatingPrep sizeMiniYieldup to 5 µg of genomic DNA - 75878-324Soil DNA Extraction KitSupplier Number: IB47802Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.75878-324DescriptionSoil DNA Extraction KitTarget moleculeDNARefine target moleculeTotal DNASample typeSoilMethod / FormatColumn (silica-based)|Bead beatingPrep sizeMiniYieldup to 5 µg of genomic DNA