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Specifications
- Description:CheckMate™ Negative Control Vectors
- Size:1 set
- Cat. No.:PAC9380
- Storage temperature:–30...–10 °C
- Supplier no.:C9380
Specifications
About this item
Powerful system for detecting protein:protein interactions in vivo.
- Powerful System for Detecting Protein:Protein Interactions In Vivo
- Mammalian system for studying interactions in cell line of choice
- Study post-translational modifications including glycosylation, phosphorylation and acylation
Two-hybrid systems are extremely powerful methods for detecting protein:protein interactions in vivo. The basis of two-hybrid systems is the modular domains found in some transcription factors: a DNA-binding domain, which binds to a specific DNA sequence, and a transcriptional activation domain, which interacts with the basal transcriptional machinery. A transcriptional activation domain in association with a DNA-binding domain will promote the assembly of RNA polymerase II complexes at the TATA box and increase transcription. In the CheckMate Mammalian Two-Hybrid System the DNA-binding domain and the transcriptional activation domain, produced by separate plasmids, are closely associated when one protein (X) fused to a DNA-binding domain interacts with a second protein (Y) fused to a transcriptional activation domain. In this system, interaction between proteins X and Y results in transcription of a reporter gene.