These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

• Protein Expression Vectors with an N-terminal GST Fusion Tag
• Express in E. coli or a cell-free expression system using the T7 promoter
• Use N-terminal glutathione-S-transferase tag to purify or bind fusion proteins
• Cleave GST from protein using TEV protease

The vectors below are used for inducible expression of GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The GST tag has been successfully used to boost tagged protein solubility during E. coli expression. The Flexi Vector System is a simple, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector. pFN2A/K (GST) Flexi Vectors (Cat.# C8461, C8471) are designed for protein expression with an N-terminal GST tag in E. coli and T7 cell-free expression systems. Note: Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.