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Specifications
- Size:20 μg
- Cat. No.:PAP2241
- Storage temperature:Less than –65 °C
- Supplier no.:P2241
Specifications
About this item
Used as a standard cloning vector and as a template for in vitro transcription.
- Standard Cloning Vector and Template for In vitro Transcription
- Blue/white screening to easily identify recombinant clones
- Multiple cloning site provides a convenient selection of restriction sites for cloning
The pGEM-5Zf(+) Vector serves as a standard cloning vector and as a template for in vitro transcription. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI.