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- Description:EZ4U Cell Proliferation Assay
- Size:1 kit
- Environmentally Preferable:
- Cat. No.:102993-994
- Supplier no.:BI-5000
EZ4U (Nonradioactive Cell Proliferation and Cytoxicity) ELISA from sm Inc
- Dynamic Range: 15 -960 U/mL
- Sensitivity: 1 U/mL
- Incubation: 2 hours
Proliferation assays are widely used in cell biology for the study of growth factors, cytokines, nutrients and for the screening of cytotoxic or chemotherapeutic agents. There are several ways to determine the number of cells either by microscopic inspection, or by the use of an electronic particle counter, indirectly by measuring the incorporation of radioactive precursors, quantitating total protein with chromogenic dyes, or by measuring metabolic activity of cellular enzymes. The most common assay for cell proliferation is the incorporation of 3H‑thymidine into cellular DNA. The 3H-thymidine assay is, however, labour intensive as it requires the removal of excess, unincorporated label by using some method of cell harvesting before measurement. In 1956, the first paper was published on the use of tetrazolium salts as indicators of cell viability. The method was based on the finding that living cells are capable to reduce slightly or uncoloured tetrazolium salts into intensely coloured formazan derivatives. This reduction process requires functional mitochondria, which are inactivated within a few minutes after cell death.